Wassil Nowicky
Ukrainian Anti-Cancer Institute, Austria
Title: Affinity of the anti-cancer preparation NSC631570 (Ukrain) to the cancer cells
Biography
Biography: Wassil Nowicky
Abstract
It is well known all living cells have a membrane potential of about -60 to –100mV. The negative sign of the membrane potential indicates that the inside surface of the cell membrane is relatively more negative than the immediate exterior surface of the cell membrane. Researchers at the University of Natural Resources and Life Sciences, Vienna (Austria) revealed human osteosarcoma cells to have a high capacity to absorb NSC631570 while absorption in normal endothelial cells was considerable lower under the same experimental conditions. This selective uptake of NSC631570 in the cancer cells has been confirmed due to its unique prop-erty to auto fluorescence under UV light. A study examined how NSC631570 affects the electro kinetic potential (EKP) of malignant and benign cells. The EKP of the Ehrlich’s carcinoma cells dropped after incubation with NSC631570. The EKP decrease of normal cells was less pronounced. The cytotoxic effect of NSC631570 was examined on two primary pancreatic cancer cell lines (PPTCC), fibroblasts from ductal pancreatic cancer tissue samples (F-PDAC) and an immortalized ductal epithelial pancreas cell line (HPNE). Cytotoxicity was established by means of Cell Titer 96 kit. In the tests with primary pancreatic cancer cell lines the modulation of NSC631570 absorption in the medium was determined with the help of the fluorescence of NSC631570 under UV light. The fluorescence test showed that carcinoma cells absorbed more of the preparation than fibroblasts and normal epithelial cells. The selective accumulation of NSC631570 in the cancer cells explains its favorable safety profile and high therapeutic index of 1250.